LOCUS pDONR™/Zeo DISCLAIMER Certain terms are trademarks or registered trademarks of Invitrogen Corporation. See "Intellectual Property" in the Help file for more information. FEATURES Location/Qualifiers misc_feature complement(268..295) /note="rrnB T2 transcription termination sequence (c)" misc_feature complement(427..470) /note="rrnB T1 transcription termination sequence (c)" primer_bind 537..552 /note="M13 Forward (-20) priming site" misc_recomb 570..668 /label=attL1 /note="attL1" misc_recomb complement(2606..2702) /label=attL2 /note="attL2 (c)" misc_signal complement(2717..2736) /note="T7 Promoter/priming site (c)" primer_bind 2744..2760 /note="M13 Reverse priming site" gene 2873..3682 /note="Kanamycin resistance gene" rep_origin 3803..4476 /note="pUC origin" vector join(2614..4479,1..651) /source="pDONR%99221" /type="Donor Vector" misc_feature 669..691 /note="TEV site" source 692..2599 /organism="Homo sapiens" /mol_type="mRNA" /db_xref="taxon:9606" /clone="MGC:31939 IMAGE:5020098" /tissue_type="Muscle, rhabdomyosarcoma" /clone_lib="NIH_MGC_17" /lab_host="DH10B-R" /note="Vector: pOTB7" gene 692..2599 /gene="HSPA5" /gene_synonym=BIP /gene_synonym=MIF2 /db_xref="GeneID:3309" /db_xref="HGNC:5238" /db_xref="MIM:138120" CDS 692..2599 /dnas_title="heat shock 70kDa protein 5 (glucose-regulated protein, 78kDa)" /gene="HSPA5" /gene_synonym=BIP /gene_synonym=MIF2 /codon_start=1 /product="heat shock 70kDa protein 5 (glucose-regulated protein, 78kDa)" /protein_id="AAH20235.1" /db_xref="GI:18044381" /db_xref="GeneID:3309" /db_xref="HGNC:5238" /db_xref="MIM:138120" /translation="MKLSLVAAMLLLLSAARAEEEDKKEDVGTVVGIDLGTTYSCVGV FKNGRVEIIANDQGNRITPSYVAFTPEGERLIGDAAKNQLTSNPENTVFDAKRLIGRT WNDPSVQQDIKFLPFKVVEKKTKPYIQVDIGGGQTKTFAPEEISAMVLTKMKETAEAY LGKKVTHAVVTVPAYFNDAQRQATKDAGTIAGLNVMRIINEPTAAAIAYGLDKREGEK NILVFDLGGGTFDVSLLTIDNGVFEVVATNGDTHLGGEDFDQRVMEHFIKLYKKKTGK DVRKDNRAVQKLRREVEKAKRALSSQHQARIEIESFYEGEDFSETLTRAKFEELNMDL FRSTMKPVQKVLEDSDLKKSDIDEIVLVGGSTRIPKIQQLVKEFFNGKEPSRGINPDE AVAYGAAVQAGVLSGDQDTGDLVLLDVCPLTLGIETVGGVMTKLIPRNTVVPTKKSQI FSTASDNQPTVTIKVYEGERPLTKDNHLLGTFDLTGIPPAPRGVPQIEVTFEIDVNGI LRVTAEDKGTGNKNKITITNDQNRLTPEEIERMVNDAEKFAEEDKKLKERIDTRNELE SYAYSLKNQIGDKEKLGGKLSSEDKETMEKAVEEKIEWLESHQDADIEDFKAKKKELE EIVQPIISKLYGSAGPPPTGEEDTAEKDEL" misc_feature 692..2599 /note="HSPA5 coding region" ORIGIN 1 CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA 61 TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA 121 GCGCCCAATA CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAGCTGGCA 181 CGACAGGTTT CCCGACTGGA AAGCGGGCAG TGAGCGCAAC GCAATTAATA CGCGTACCGC 241 TAGCCAGGAA GAGTTTGTAG AAACGCAAAA AGGCCATCCG TCAGGATGGC CTTCTGCTTA 301 GTTTGATGCC TGGCAGTTTA TGGCGGGCGT CCTGCCCGCC ACCCTCCGGG CCGTTGCTTC 361 ACAACGTTCA AATCCGCTCC CGGCGGATTT GTCCTACTCA GGAGAGCGTT CACCGACAAA 421 CAACAGATAA AACGAAAGGC CCAGTCTTCC GACTGAGCCT TTCGTTTTAT TTGATGCCTG 481 GCAGTTCCCT ACTCTCGCGT TAACGCTAGC ATGGATGTTT TCCCAGTCAC GACGTTGTAA 541 AACGACGGCC AGTCTTAAGC TCGGGCCCCA AATAATGATT TTATTTTGAC TGATAGTGAC 601 CTGTTCGTTG CAACACATTG ATGAGCAATG CTTTTTTATA ATGCCAACTT TGTACAAAAA 661 AGCAGGCTct gaaaacttgt actttcaagg cgaggaggag gacaagaagg aggacgtggg 721 cacggtggtc ggcatcgacc tggggaccac ctactcctgc gtcggcgtgt tcaagaacgg 781 ccgcgtggag atcatcgcca acgatcaggg caaccgcatc acgccgtcct atgtcgcctt 841 cactcctgaa ggggaacgtc tgattggcga tgccgccaag aaccagctca cctccaaccc 901 cgagaacacg gtctttgacg ccaagcggct catcggccgc acgtggaatg acccgtctgt 961 gcagcaggac atcaagttct tgccgttcaa ggtggttgaa aagaaaacta aaccatacat 1021 tcaagttgat attggaggtg ggcaaacaaa gacatttgct cctgaagaaa tttctgccat 1081 ggttctcact aaaatgaaag aaaccgctga ggcttatttg ggaaagaagg ttacccatgc 1141 agttgttact gtaccagcct attttaatga tgcccaacgc caagcaacca aagacgctgg 1201 aactattgct ggcctaaatg ttatgaggat catcaacgag cctacggcag ctgctattgc 1261 ttatggcctg gataagaggg agggggagaa gaacatcctg gtgtttgacc tgggtggcgg 1321 aaccttcgat gtgtctcttc tcaccattga caatggtgtc ttcgaagttg tggccactaa 1381 tggagatact catctgggtg gagaagactt tgaccagcgt gtcatggaac acttcatcaa 1441 actgtacaaa aagaagacgg gcaaagatgt caggaaagac aatagagctg tgcagaaact 1501 ccggcgcgag gtagaaaagg ccaaacgggc cctgtcttct cagcatcaag caagaattga 1561 aattgagtcc ttctatgaag gagaagactt ttctgagacc ctgactcggg ccaaatttga 1621 agagctcaac atggatctgt tccggtctac tatgaagccc gtccagaaag tgttggaaga 1681 ttctgatttg aagaagtctg atattgatga aattgttctt gttggtggct cgactcgaat 1741 tccaaagatt cagcaactgg ttaaagagtt cttcaatggc aaggaaccat cccgtggcat 1801 aaacccagat gaagctgtag cgtatggtgc tgctgtccag gctggtgtgc tctctggtga 1861 tcaagataca ggtgacctgg tactgcttga tgtatgtccc cttacacttg gtattgaaac 1921 tgtgggaggt gtcatgacca aactgattcc aaggaacaca gtggtgccta ccaagaagtc 1981 tcagatcttt tctacagctt ctgataatca accaactgtt acaatcaagg tctatgaagg 2041 tgaaagaccc ctgacaaaag acaatcatct tctgggtaca tttgatctga ctggaattcc 2101 tcctgctcct cgtggggtcc cacagattga agtcaccttt gagatagatg tgaatggtat 2161 tcttcgagtg acagctgaag acaagggtac agggaacaaa aataagatca caatcaccaa 2221 tgaccagaat cgcctgacac ctgaagaaat cgaaaggatg gttaatgatg ctgagaagtt 2281 tgctgaggaa gacaaaaagc tcaaggagcg cattgatact agaaatgagt tggaaagcta 2341 tgcctattct ctaaagaatc agattggaga taaagaaaag ctgggaggta aactttcctc 2401 tgaagataag gagaccatgg aaaaagctgt agaagaaaag attgaatggc tggaaagcca 2461 ccaagatgct gacattgaag acttcaaagc taagaagaag gaactggaag aaattgttca 2521 accaattatc agcaaactct atggaagtgc aggccctccc ccaactggtg aagaggatac 2581 agcagaaaaa gatgagttgT AGGACCCAGC TTTCTTGTAC AAAGTTGGCA TTATAAGAAA 2641 GCATTGCTTA TCAATTTGTT GCAACGAACA GGTCACTATC AGTCAAAATA AAATCATTAT 2701 TTGCCATCCA GCTGATATCC CCTATAGTGA GTCGTATTAC ATGGTCATAG CTGTTTCCTG 2761 GCAGCTCTGG CCCGTGTCTC AAAATCTCTG ATGTTACATT GCACAAGATA AAATAATATC 2821 ATCATGAACA ATAAAACTGT CTGCTTACAT AAACAGTAAT ACAAGGGGTG TTATGAGCCA 2881 TATTCAACGG GAAACGTCGA GGCCGCGATT AAATTCCAAC ATGGATGCTG ATTTATATGG 2941 GTATAAATGG GCTCGCGATA ATGTCGGGCA ATCAGGTGCG ACAATCTATC GCTTGTATGG 3001 GAAGCCCGAT GCGCCAGAGT TGTTTCTGAA ACATGGCAAA GGTAGCGTTG CCAATGATGT 3061 TACAGATGAG ATGGTCAGAC TAAACTGGCT GACGGAATTT ATGCCTCTTC CGACCATCAA 3121 GCATTTTATC CGTACTCCTG ATGATGCATG GTTACTCACC ACTGCGATCC CCGGAAAAAC 3181 AGCATTCCAG GTATTAGAAG AATATCCTGA TTCAGGTGAA AATATTGTTG ATGCGCTGGC 3241 AGTGTTCCTG CGCCGGTTGC ATTCGATTCC TGTTTGTAAT TGTCCTTTTA ACAGCGATCG 3301 CGTATTTCGT CTCGCTCAGG CGCAATCACG AATGAATAAC GGTTTGGTTG ATGCGAGTGA 3361 TTTTGATGAC GAGCGTAATG GCTGGCCTGT TGAACAAGTC TGGAAAGAAA TGCATAAACT 3421 TTTGCCATTC TCACCGGATT CAGTCGTCAC TCATGGTGAT TTCTCACTTG ATAACCTTAT 3481 TTTTGACGAG GGGAAATTAA TAGGTTGTAT TGATGTTGGA CGAGTCGGAA TCGCAGACCG 3541 ATACCAGGAT CTTGCCATCC TATGGAACTG CCTCGGTGAG TTTTCTCCTT CATTACAGAA 3601 ACGGCTTTTT CAAAAATATG GTATTGATAA TCCTGATATG AATAAATTGC AGTTTCATTT 3661 GATGCTCGAT GAGTTTTTCT AATCAGAATT GGTTAATTGG TTGTAACACT GGCAGAGCAT 3721 TACGCTGACT TGACGGGACG GCGCAAGCTC ATGACCAAAA TCCCTTAACG TGAGTTACGC 3781 GTCGTTCCAC TGAGCGTCAG ACCCCGTAGA AAAGATCAAA GGATCTTCTT GAGATCCTTT 3841 TTTTCTGCGC GTAATCTGCT GCTTGCAAAC AAAAAAACCA CCGCTACCAG CGGTGGTTTG 3901 TTTGCCGGAT CAAGAGCTAC CAACTCTTTT TCCGAAGGTA ACTGGCTTCA GCAGAGCGCA 3961 GATACCAAAT ACTGTTCTTC TAGTGTAGCC GTAGTTAGGC CACCACTTCA AGAACTCTGT 4021 AGCACCGCCT ACATACCTCG CTCTGCTAAT CCTGTTACCA GTGGCTGCTG CCAGTGGCGA 4081 TAAGTCGTGT CTTACCGGGT TGGACTCAAG ACGATAGTTA CCGGATAAGG CGCAGCGGTC 4141 GGGCTGAACG GGGGGTTCGT GCACACAGCC CAGCTTGGAG CGAACGACCT ACACCGAACT 4201 GAGATACCTA CAGCGTGAGC TATGAGAAAG CGCCACGCTT CCCGAAGGGA GAAAGGCGGA 4261 CAGGTATCCG GTAAGCGGCA GGGTCGGAAC AGGAGAGCGC ACGAGGGAGC TTCCAGGGGG 4321 AAACGCCTGG TATCTTTATA GTCCTGTCGG GTTTCGCCAC CTCTGACTTG AGCGTCGATT 4381 TTTGTGATGC TCGTCAGGGG GGCGGAGCCT ATGGAAAAAC GCCAGCAACG CGGCCTTTTT 4441 ACGGTTCCTG GCCTTTTGCT GGCCTTTTGC TCACATGTT //